KMID : 0811720180220040369
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Korean Journal of Physiology & Pharmacology 2018 Volume.22 No. 4 p.369 ~ p.377
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Isoliquiritigenin attenuates spinal tuberculosis through inhibiting immune response in a New Zealand white rabbit model
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Wang Wenjing
Yang Baozhi Cui Yong Zhan Ying
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Abstract
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Spinal tuberculosis (ST) is the tuberculosis caused by Mycobacterium tuberculosis (Mtb) infections in spinal curds. Isoliquiritigenin (4,2¡¯,4¡¯-trihydroxychalcone, ISL) is an anti-inflammatory flavonoid derived from licorice (Glycyrrhiza uralensis), a Chinese traditional medicine. In this study, we evaluated the potential of ISL in treating ST in New Zealand white rabbit models. In the model, rabbits (n=40) were infected with Mtb strain H37Rv or not in their 6th lumbar vertebral bodies. Since the day of infection, rabbits were treated with 20 mg/kg and 100 mg/kg of ISL respectively. After 10 weeks of treatments, the adjacent vertebral bone tissues of rabbits were analyzed through Hematoxylin-Eosin staining. The relative expression of Monocyte chemoattractant protein-1 (MCP-1/CCL2), transcription factor ¥êB (NF-¥êB) p65 in lymphocytes were verified through reverse transcription quantitative real-time PCR (RT-qPCR), western blotting and enzyme-linked immunosorbent assays (ELISA). The serum level of interleukin (IL)-2, IL-4, IL-10 and interferon ¥ã (IFN-¥ã) were evaluated through ELISA. The effects of ISL on the phosphorylation of I¥êB¥á, IKK¥á/¥â and p65 in NF-¥êB signaling pathways were assessed through western blotting. In the results, ISL has been shown to effectively attenuate the granulation inside adjacent vertebral tissues. The relative level of MCP-1, p65 and IL-4 and IL-10 were retrieved. NF-¥êB signaling was inhibited, in which the phosphorylation of p65, I¥êB¥á and IKK¥á/¥â were suppressed whereas the level of I¥êB¥á were elevated. In conclusion, ISL might be an effective drug that inhibited the formation of granulomas through downregulating MCP-1, NF-¥êB, IL-4 and IL-10 in treating ST.
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KEYWORD
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Granuloma, Inflammatory cytokines, MCP-1, NF-¥êB, Spinal tuberculosis
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